Characterization of a Yellowstone Hot Spring Microbial Community by 5S rRNA Sequences
Applied and Environmental Microbiology, 1985
The microorganisms inhabiting a 91°C hot spring in Yellowstone National Park were characterized by
sequencing 5S rRNAs isolated from the mixed, natural microflora without cultivation. By comparisons of these
sequences with reference sequences, the phylogenetic relationships of the hot spring organisms to better
characterized ones were established. Quantitation of the total 5S-sized rRNAs revealed a complex microbial
community of three dominant members, a predominant archaebacterium affiliated with the sulfur-metabolizing
(dependent) branch of the archaebacteria, and two eubacteria distantly related to Thermus spp. The
archaebacterial and the eubacterial 5S rRNAs each constituted about half the examined population.
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